Report: TransPot Consortium Meeting & the 26th Meeting of the EAU Section of Urological Research My name is Ingrid Tomljanovic, I am a Marie Skłodowska-Curie early stage researcher (ESR) within the EU-funded project TransPot (the Translational Research Network for Prostate Cancer), working at the R&D department of GenomeScan. TransPot is a European research training network aimed at deepening the understanding…
Translational Research Network for Prostate Cancer (TransPot) Today starts Movember (a combination of the English diminutive word for moustache, “mo”, and “November”), an annual event involving the growing of moustaches during the month of November to raise awareness of men’s health issues, such as prostate cancer, testicular cancer and men’s suicide. Prostate cancer is one of the most prevalent cancer…
Improvements in Gene Panel and Whole Exome sequencing Molecular barcoding refers to the attachment of a unique molecular sequence (eg. ATGCCA) to each individual DNA fragment in a sample. This ensures that in downstream analysis, unique sequence reads can be separated from those originating as a result of PCR duplication. A common practice regarding these PCR duplicates is to search…
Transcription profiles give accurate images of the biological processes that influence cell fates. However, library construction can introduce bias at multiple steps. For example, PCR amplification increases the number of cDNA molecules to an amount sufficient for sequencing, but also stochastically introduces errors and amplifies different molecules with unequal probabilities. The sequencing reads that have been created from the same…
If you start analyzing your gene-expression data set, many questions come to mind that can seriously affect the outcome. What if any of the PCR steps during library prep (Figure 1) has introduced an error? It might have led to a base change, which I could incorrectly score as a mutation. Did a PCR-bias favor amplification of some of the…
Hi everybody! I’m Mario Cangiano, an Early stage researcher (ESR) within the EU funded project TransPot (the Translational Research Network for Prostate Cancer), working at the R&D department of GenomeScan. Transpot is a multi-center research program designed to tackle aggressive prostate cancer (PCa) and to train 11 ESR in the field of systems biology, namely the holistic representation of biological…
Some highlights from SMRT Leiden 2019 Innovation in long-read sequencing applications SMRT Leiden – 7th -9th of May 2019 Arnoud Schmitz – PacBio® expert at GenomeScan The yearly PacBio meeting brings together knowledgeable scientists who share the same enthusiasm for long reads. This two-days event is the perfect place to discuss innovative ideas, visions and meet with inspiring peoples. Here…
Recent Comments