Epigenetics

The leading service provider for (hydroxy)methylation studies in Europe

Next Level Epigenetics

GenomeScan sets the standard for sharply priced array and NGS methylation sequencing products

Epigenetic services using Micro-array and Next Generation Sequencing

Illumina released the sucessor of the highly popular 450K array in 2016. The MethylationEPIC ‘850K’ has all the benefits of the 450K bead-array, but with double the amount of interrogated loci. This array combines a unique wealth of data with low costs per sample and easy interpretation. These arrays can also be used to detect hydroxymethylated loci. We can selectively detect this DNA modification, providing an excellent tool for researchers in the field of cellular differentiation, neuronal development and cancer research.

An even more detailed data-set can be achieved by two Next Generation Sequencing services: Reduced Representation Bisulfite Sequencing (RRBS) and Whole Genome Bisulfite sequencing (WGBS). While WGBS interrogates the whole genomic sequence, for RRBS specifically the methylated loci are selected and analyzed.

Do you have questions, or do you need free expert advise? Please contact our product specialists!

MethylationEPIC array

The newest bead array of Illumina, which remains the most-used tool in methylation research. Experts in the field have recently updated the content, so even recently described clinically relevant methylation sites are incorporated.

Benefits of the 850K MethylationEPIC BeadArray

  • Expert-selected coverage of 850,000 loci thoughout the genome
  • Includes 99% of RefSeq genes, 95% of CpG islands and high coverage of enhancers
  • Quantitative analysis at single nucleotide resolution
  • Includes non-CpG methylated sites identified in human stem cells (CHH sites)
  • Focus on differentially methylated sites in tumor versus normal
  • Covers over 90% of the original content from the Infinium HumanMethylation450K

Our full service includes

  • Validated and accredited workflow
  • All experiments are performed by highly specialized researchers
  • QC measurements at multiple stages to tightly monitor sample quality
  • Comprehensive report with data visualization in MethylAID
  • Full service, but core-facility pricing due to high turnover
Whole Genome Bisulfite Sequencing (WGBS)

WGBS is the most informative method to study methylation. The whole genome is converted using Bisulfite treatment.

With this method, non-methylated cytosine (C) nucleotides are converted to uracil (U) and read as thymine (T) when sequenced. Methylated cytosines protected from conversion are still read as cytosine (C). Because no a priori selection is made, it gives the most detailed methylation status throughout the whole genomic DNA.

Benefits of WGBS

  • Full resolution of the entire genome
  • No selection-bias
  • Applicable to all species

Our full service includes

  • Validated and accredited workflow
  • Thorough advice and guidance of our specialists
  • All experiments are performed by our highly specialized researchers
  • QC measurements at multiple stages to tightly monitor sample quality
  • Optional: extensive data analysis with comprehensive report
Reduced Representation Bisulfite Seq (RRBS)

Illumina solely provides methylation arrays for human samples. A custom approach is also necessary for those interested in CpGs outside the 850K target list. Our scientists assessed many targeted sequencing approaches to select the most robust method to measure methylated loci. RRBS combines the wealth of unbiased data that only NGS can provide, with relatively low costs.

Benefits of RRBS

  • Selection of methylated sites by MspI restriction analysis
  • Unbiased approach – no fixed content
  • Applicable to all species

Our full service includes

  • Validated workflow within our ISO 17025 accreditation scope
  • Thorough advice and guidance of our specialists
  • All experiments are performed by our highly specialized researchers
  • QC measurements at multiple stages to tightly monitor sample quality

Choose the most optimal project method for your project

Methylation  Hydroxy methylation
Micro-array analysis Reduced Representation Bisulfite Sequencing (RRBS) Whole Genome Bisulfite Sequencing (WGBS) Micro-array analysis
Organisms Human only All All Human only
Tissue of highest abundance All All All Neuronal cells, stem cells, cancer tissue
Methylated targets 850,000 selected loci All All 850,000 selected loci
Validated and within ISO 17025 accreditation scope
Applicable for FFPE samples
Costs Very budget friendly Most economical option for sequencing Best option for selected samples Best option to study hmC’ and mC’
Benefits Best option for a focused approach Best unbiased approach for methylation research Most informative method Sole method to discriminate between hmC’ and mC’
Most used for EWAS studies or large cohorts Broad analysis for cohorts Analysis of selected samples Large cohorts or EWAS studies for neuronal diseases
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