This is why you should use total transcriptome:
Standard gene-expression analysis is performed on protein-coding RNA, since this provides you the fastest and affordable method to visualize gene-expression. With Total transcriptome, you extend your vision towards all transcripts, including long non-coding RNA (lncRNA) or other regulatory RNA species.
Total RNA sequencing helps you to overcome common causes for RNA degradation, such as FFPE-fixation and laser-capture methods. Additionally, precious samples from a cohort might not be handled optimally, blocking the only source to obtain transcriptome profiles for your study. Our scientists have set up validated procedures to analyze these challenging samples. This includes clinical-grade sample isolation from FFPE material and tailored data-analysis.
A ribosomal RNA (rRNA) depletion step is usually required, since the 16S and 18S ribosomal subunits make up ~60-90% of all RNA species present in your sample. You make better use of the sequencing space by removing them at the sample preparation phase. What remains is messenger RNA, and other types of RNA such as long non-coding RNAs (lncRNAs).