Single cell sequencing

Unparalleled perspective on the biology of single cells

 

Single-cell Gene-expression analysis has been chosen by SCIENCE editors as “Breakthrough of the Year 2018”. Now, this technique has become available for any researcher to study the processes behind cellular development and disease.

Standard RNA-seq measures average gene-expression levels, which is inadequate for heterogenous samples. Single cell sequencing does not require sorting your cells into small populations based on known markers. It allows you to administer any cell population – as a whole or pre-sorted – and create gene-expression profiles on each cell individually. Unsupervised clustering algorithms then visualize your cells in plots, placing cells with similar expression profiles together. You can clearly discern between cell types and study the heterogeneity within those clusters. Visualizing cell-to-cell variability has never been more attainable.

Mask Group 38 Service page   Single Cell sequencing

Benefits of Single cell sequencing:

Rare cell populations 01 Service page   Single Cell sequencing

...View more

Investigate rare cell populations such as stem cells, primary cells or cells refractory to treatment

View less

Cell regulation 01 Service page   Single Cell sequencing

...View more

Monitor cellular regulation and differentiation processes

View less

Drug response 01 Service page   Single Cell sequencing

...View more

Observe drug responses within cell populations and/or over time

View less

New biomarkers 01 Service page   Single Cell sequencing

...View more

Discover new biomarkers

View less

A group of people with the appropriate skills work with you on your project:

Getting started 1 01 Service page   Single Cell sequencing

Single cell sequencing poses unique challenges since it requires freshly dissociated, viable cell suspensions.

...View more

Having in-house expertise in cell-handling as well as NGS sample preparation, is crucial. Equally important is the guidance before the start of your project. A PhD-level project manager will give expert advice and collaborates with you to optimize the workflow for your specific cell culture, tissue or organ. GenomeScan has set up a streamlined workflow and logistics strategy to optimally preserve the biological relevance.

View less

Methodology 2 01 Service page   Single Cell sequencing

GenomeScan has selected the Chromium instrument of 10X Genomics as the most reproducible and cost-effective method for data-generation.

...View more

Individual cells are loaded into Gel Bead-In EMulsions droplets (GEMs) to uniquely barcode 500 to 50.000 cells per sample.

Figure methodology Service page   Single Cell sequencing  

View less

Ready for interpretation 3 01 Service page   Single Cell sequencing

Your single cell gene-expression data is directly accessible for interpretation in an interactive visualization tool: the Chromium Loupe browser.

...View more

The raw RNA-seq output is processed by our high capacity servers by the Cell Ranger analysis pipeline. This generates all files for further analysis, that you can directly read from the Hard Disk that you receive. In the Loupe browser, you can further explore gene expression differences by creating groups, using t-SNE cluster plots and gene-expression tables.

Figure interpreation Service page   Single Cell sequencing

View less

Detailed data analysis 4 01 Service page   Single Cell sequencing

For comparative analysis such as time-series or drug treatments, additional data-analysis is required.

...View more

Our experienced bioinformaticians have adapted analysis methods to identify common sources of variation between datasets and to perform correction, so that statistically relevant data is reported.

You receive a detailed report of the transcriptomic changes between cell-clusters and samples, including leading to the fastest way to answering your research question. Alternatively, we can provide expert guidance for BioIT specialists that have experience in data-analysis using R-packages.

Figure data analysis Service page   Single Cell sequencing

View less

Pricing

 

Competitive pricing for
unmatched quality

Request pricing 1 Service page   Single Cell sequencing

Video

 

GenomeScan uses 10x Genomics
Chromium single cell gene expression
solution.

Watch how it works button 1 Service page   Single Cell sequencing

Advantages

Optimization 3 Service page   Single Cell sequencing

Optimization of the experimental design with a PhD-level project manager, including pre-sample treatment and logistics

Industry leading Service page   Single Cell sequencing

Your samples will be sequenced on the industry leading NovaSeq 6000 to reduce time and costs

Interactive analysis Service page   Single Cell sequencing

Interactive analysis report with t-SNE cluster plots and fold change tables

Expert Service page   Single Cell sequencing

Expert bioinformaticians can provide full support on comparative data-analysis

Accredited services Service page   Single Cell sequencing

The GenomeScan laboratory can provide ISO/IEC 17025, ISO 15189, and/or G(C)LP accredited services

We have summarized key information about our single cell sequencing service into a product specification sheet, which you can download here.

Discuss your project with Andre

We have years of experience in the field. Talk with us about best practices and how we can help your research.

Photo André rond 01 Service page   Single Cell sequencing

Andre Wijfjes

Manager Customer Support

+31 71 568 1036

Gene expression analysis Service page   Single Cell sequencing
Total Transcriptome Service page   Single Cell sequencing
Small RNAseq Service page   Single Cell sequencing
Ultra low transcriptomics Service page   Single Cell sequencing
Group 31 Service page   Single Cell sequencing
Group 30 Service page   Single Cell sequencing
Group 33 Service page   Single Cell sequencing
Sans titre 4 Service page   Single Cell sequencing

Let’s get the conversation started for your next NGS project.

Group 123 Service page   Single Cell sequencing
Please either fill in this form or email us directly at info@genomescan.nl
and we will get in touch with you to discuss your requirements.