We offer innovative NGS and microarray-based solutions for genetic research and diagnostics
The major benefits of working with GenomeScan:
While sharing our knowledge and experience, we keep listening to understand your constraints and your needs. Our commitment and dedication keep us on your team long after completion of your project with excellent customer care.
With scientists and technicians from various disciplines, we broaden our network. They can be considered as extra members of your team.
Our close contacts with technology providers and numerous collaborations with academic facilities give you access to the most advanced sequencing methodologies.
We are committed to complete your project on time, on budget and according to stringent quality criteria.
GenomeScan offers customizable sequencing solutions covering a great variety of DNA and RNA applications:
| DNA | RNA | |
| Whole Genome Sequencing (WGS) | Mitochondrial DNA (mtDNA) | RNA-Seq using Poly-A selection ** (Gene-expression profiling) |
| Targeted Resequencing | Microbiome 16S rRNA Sequencing | RNA-Seq using rRNA reduction ** (Total transcriptome) |
| Whole Exome Sequencing (WES) | Whole Microbiome Sequencing | Small RNA-Seq |
| ChIP-Sequencing/complexity reduction | Methylation EPIC BeadChip (850K) | Low-input RNA-Seq |
| Whole Genome Bisulfite Sequencing (WGBS) | PacBio Sequencing | Single cell RNA-Seq |
You can find more information on these applications in the DNA and RNA tabs. Alternatively, you can download the Sample Submission Guide with an overview of key information to initiate your project.
All samples should be submitted in ≥ 15 μl volume
| Application | Input material | Optimal amount (per sample) | Concentration range | Turnaround time* |
| Whole Genome Sequencing (WGS) | Purified gDNA | Standard: >10 ng PCR free protocol: >100ng | 5-50 ng/μl | 2-3 weeks |
| Targeted Resequencing | Purified gDNA/ Amplicon | Standard: >10 ng PCR free protocol: >100ng | 5-50 ng/μl | 2-3 weeks |
| Whole Exome Sequencing (WES) | Purified gDNA | 500 ng | 50-200 ng/μl | 2-3 weeks |
| ChIP-Sequencing/complexity reduction | Enriched DNA | Standard: >10 ng PCR free protocol: >100ng | >2 ng/μl | 2-3 weeks |
| Whole Genome Bisulfite Sequencing (WGBS) | Purified gDNA | 100 ng | >10 ng/μl | 3-4 weeks |
| Mitochondrial DNA (mtDNA) | Purified gDNA | 250-500 ng | 5-50 ng/μl | 2-3 weeks |
| Microbiome16S rRNA Sequencing | Purified gDNA | 1-100 ng ** | >2 ng/μl | 2-3 weeks |
| Whole Microbiome Sequencing | Purified gDNA | 250-500 ng | 5-50 ng/μl | 2-3 weeks |
| Methylation EPIC BeadChip (850K) | Purified gDNA | 250 ng | 25 ng/μl | 3-4 weeks |
| PacBio Sequencing | Purified gDNA/ Amplicon | 500 ng | 500 ng | 2-6 weeks |
* Turnaround time (TAT) depends on project size and starts after successful sample
** Depending on sample origin
All samples should be submitted in ≥ 15 μl volume
| Application | Input material | Optimal amount (per sample) | Concentration range | Turnaround time* |
|
RNA-Seq using Poly-A selection ** (Gene-expression profiling) |
Purified RNA |
≥100 ng |
10-50 ng/μl |
3-4 weeks |
|
RNA-Seq using rRNA reduction ** (Total transcriptome) |
Purified RNA |
≥ 30 ng |
3-50 ng/μl |
3-4 weeks |
|
Small RNA-Seq |
Purified RNA or equivalent of enriched small RNA |
1000 ng or enriched equivalent |
250-350 ng/μl |
3-4 weeks |
|
Low-input RNA-Seq |
Purified RNA (or cells) |
1 ng |
>0.1 ng/μl |
3-4 weeks |
|
Single cell RNA-Seq |
Methanol fixed cells |
1.000 cells |
1.000-10.000 cells |
3-4 weeks |
* Turnaround time (TAT) depends on project size and starts after successful sample QC
** Optimal input for RNA-Seq based on 30% or less PCR duplicates
The table below specifies the input requirement for RNA samples. Be aware that these recommendations were determined using a high purity and quality reference sample. Samples of sub-optimal quality will require more material to achieve similar numbers.
| Input | Optimal | Recommended | Minimum |
|
RNA-Seq using Poly-A selection * (Gene-expression profiling) |
Purified RNA |
≥100 ng |
10-50 ng/μl
|
|
RNA-Seq using rRNA reduction * (Total transcriptome) |
Purified RNA |
≥ 30 ng |
3-50 ng/μl |
|
Small RNA-Seq |
Purified RNA or equivalent of enriched small RNA |
1000 ng or enriched equivalent |
250-350 ng/μl |
- + Our Services
-
GenomeScan offers customizable sequencing solutions covering a great variety of DNA and RNA applications:
DNA RNA Whole Genome Sequencing (WGS) Mitochondrial DNA (mtDNA) RNA-Seq using Poly-A selection ** (Gene-expression profiling) Targeted Resequencing Microbiome 16S rRNA Sequencing RNA-Seq using rRNA reduction ** (Total transcriptome) Whole Exome Sequencing (WES) Whole Microbiome Sequencing Small RNA-Seq ChIP-Sequencing/complexity reduction Methylation EPIC BeadChip (850K) Low-input RNA-Seq Whole Genome Bisulfite Sequencing (WGBS) PacBio Sequencing Single cell RNA-Seq You can find more information on these applications in the DNA and RNA tabs. Alternatively, you can download the Sample Submission Guide with an overview of key information to initiate your project.
- + DNA
-
All samples should be submitted in ≥ 15 μl volume
Application Input material Optimal amount (per sample) Concentration range Turnaround time* Whole Genome Sequencing (WGS) Purified gDNA Standard: >10 ng PCR free protocol: >100ng 5-50 ng/μl 2-3 weeks Targeted Resequencing Purified gDNA/ Amplicon Standard: >10 ng PCR free protocol: >100ng 5-50 ng/μl 2-3 weeks Whole Exome Sequencing (WES) Purified gDNA 500 ng 50-200 ng/μl 2-3 weeks ChIP-Sequencing/complexity reduction Enriched DNA Standard: >10 ng PCR free protocol: >100ng >2 ng/μl 2-3 weeks Whole Genome Bisulfite Sequencing (WGBS) Purified gDNA 100 ng >10 ng/μl 3-4 weeks Mitochondrial DNA (mtDNA) Purified gDNA 250-500 ng 5-50 ng/μl 2-3 weeks Microbiome16S rRNA Sequencing Purified gDNA 1-100 ng ** >2 ng/μl 2-3 weeks Whole Microbiome Sequencing Purified gDNA 250-500 ng 5-50 ng/μl 2-3 weeks Methylation EPIC BeadChip (850K) Purified gDNA 250 ng 25 ng/μl 3-4 weeks PacBio Sequencing Purified gDNA/ Amplicon 500 ng 500 ng 2-6 weeks * Turnaround time (TAT) depends on project size and starts after successful sample
** Depending on sample origin
- + RNA
-
All samples should be submitted in ≥ 15 μl volume
Application Input material Optimal amount (per sample) Concentration range Turnaround time* RNA-Seq using Poly-A selection ** (Gene-expression profiling)
Purified RNA
≥100 ng
10-50 ng/μl
3-4 weeks
RNA-Seq using rRNA reduction ** (Total transcriptome)
Purified RNA
≥ 30 ng
3-50 ng/μl
3-4 weeks
Small RNA-Seq
Purified RNA or equivalent of enriched small RNA
1000 ng or enriched equivalent
250-350 ng/μl
3-4 weeks
Low-input RNA-Seq
Purified RNA (or cells)
1 ng
>0.1 ng/μl
3-4 weeks
Single cell RNA-Seq
Methanol fixed cells
1.000 cells
1.000-10.000 cells
3-4 weeks * Turnaround time (TAT) depends on project size and starts after successful sample QC
** Optimal input for RNA-Seq based on 30% or less PCR duplicates
The table below specifies the input requirement for RNA samples. Be aware that these recommendations were determined using a high purity and quality reference sample. Samples of sub-optimal quality will require more material to achieve similar numbers.
Input Optimal Recommended Minimum RNA-Seq using Poly-A selection * (Gene-expression profiling)
Purified RNA
≥100 ng
10-50 ng/μl
RNA-Seq using rRNA reduction * (Total transcriptome)
Purified RNA
≥ 30 ng
3-50 ng/μl
Small RNA-Seq
Purified RNA or equivalent of enriched small RNA
1000 ng or enriched equivalent
250-350 ng/μl
Let’s get the conversation started for your next NGS project.
Please either fill in this form or email us directly at info@genomescan.nl
and we will get in touch with you to discuss your requirements.
