Small RNA-seq

Detect miRNA and small RNAs in any organism

This is why you should use small RNA-seq:

Small RNA’s are <100 nucleotides in length, and are usually non-coding RNA molecules. They have a regulatory function, mainly in RNA silencing. Gene-expression can be regulated by endogenous expression of microRNA’s (miRNA) that induce the degradation of their complementary messenger RNA.

GenomeScan’s Small RNA-Seq service allows you to identify and quantify all small RNA types, including miRNA, siRNA, snoRNA, snRNA, mtRNA, piRNA and other non-coding sequences. Our R&D team has developed an accredited method, allowing you to select the size range of the transcripts you are interested in. You can start with very little material: the total RNA input can be as low as 10 ng. With this minimal input, samples showed a concordance (R2) of 0.99 in our validation study. This opens many possibilities to analyze miRNA in liquid biopsies or tumor biopts.

In cancer research, miRNA’s are often the target of investigation. miRNA-deregulation can lead to all sorts of cancer types, where they are referred to as ‘oncomiRs’. They can also play a role in diagnosis of certain subtypes of cancers, prediction of the disease prognosis, and in stratifying patients. Even in the treatment of certain cancers, mRNA’s can be used as either a tool or a target for therapy.

In certain samples, several miRNA’s might be abundant, thereby masking the detection of other miRNA’s. Our scientists can design custom blocking oligo’s to remove these uninformative miRNAs from the library, so that less sequencing-space is needed.

See how we work with you on your project:

  • Optimal RNA input: 100 ng / sample
  • Ideally deliver ≥250 ng RNA to allow for QC and normalization
  • Minimally validated RNA input: 10 ng
  • For RNA quality of RIN / RQN ≥7
  • Sequencing of miRNA’s
  • Quality score Q30 of ≥75% for PE 150 reads
  • Turn-around time: 4-6 weeks
  • Sequencing on NovaSeq 6000 PE150 or NextSeq 500 PE38
  • Data required for analyzing smallRNAs: generally 20 million reads

The specifications of the data-set that you can expect are listed in your personal quotation. 

...View more

Generally, the sequencing quality score (Q score; ≥30 represents high quality) must be ≥75% for PE 150 reads (Illumina’s official guarantee ≥75%). Our average score of 2018 was
Q30 ≥ 90%.

View less

Discuss your project with Raymond

We have years of experience in sequencing RNAs. Talk with us about best practices and how we can help your research.

Raymond Egging

Director Marketing & Sales Diagnostics/R&D

+31 71 568 1050