Targeted sequencing of the methylome
If you need to assess as many loci as possible without spending your budget on large data-files and complicated data-analysis, GenomeScan can provide the SeqCap Epi (Roche) application. This technique uses hybridization capture usually carefully designed probes, reducing the part of the genome that must be analysed to solely the relevant loci.
Select only epigenomic regions of interest
With SeqCap Epi you can choose your own content or select the CpGiant Enrichment Kit. This off-the-shelf probe-set interrogates over 5.5 million methylation sites per sample at single-nucleotide resolution. This kit can serve as a screening tool to identify specific regions in the genome for methylation variation assessment. Capturing of known differentially methylated regions vastly reduces the sequencing output required to analyze the whole methylation landscape, compared to whole genome sequencing. Furthermore, it enables you to observe the methylation changes on both strands, while you can still study the SNPs in the targeted regions.
Only 10 ng of genomic DNA is required
Our ISO/IEC 17025 (L518) accredited workflow starts with only 10 ng of genomic DNA, due to a proprietary sample preparation method. This is a dramatic reduction of the 1000 ng that is recommended by the manufacturer. Cell-sorted DNA or serum- and plasma-derived cell free (cf)DNA can easily be analysed with this lowered input quantity.