You can now truly understand cell-to-cell variability. Bypass the need for sorting and analysis of each single cell types separately with single cell sequencing. Employ the power of unsupervised clustering based on the gene-expression of each individual cell.

Do you get the whole picture?

Bulk RNA-seq measures average gene-expression levels, which is insufficient for heterogenous samples. For single cell sequencing, sorting and analyzing all cell types separately based on known markers is not necessary. Unsupervised clustering divides the cells of your tissue or biological sample based on the gene-expression levels of the individual cells. You can now truly understand the cell-to-cell variability.

workflow scRNAseq Single cell RNA seq

Do you get the whole picture?

Bulk RNA-seq measures average gene-expression levels, which is insufficient for heterogenous samples. For single cell sequencing, sorting and analyzing all cell types separately based on known markers is not necessary. Unsupervised clustering divides the cells of your tissue or biological sample based on the gene-expression levels of the individual cells. You can now truly understand the cell-to-cell variability.

Expertise

Single cell sequencing poses unique challenges since it requires freshly dissociated, viable cell suspensions. Our single cell specialists have set up a streamlined workflow and logistics strategy to preserve the biological relevance. Our BioIT experts can provide all the support to analyze these highly complex data-sets.

Don’t miss a single cell!

GenomeScan collaborates with 10x Genomics and Illumina to offer the most robust single cell analysis method on the market. Validation tests have shown that the reproducibility in combination with high throughput is optimal to run clinical-grade projects. Individual cells are loaded into Gel Bead-In EMulsions droplets (GEMs) to uniquely barcode 500 to 50.000 cells per sample.

Single-cell RNA-seq analysis at GenomeScan

  • A dedicated Project Specialist will optimize your experimental design, pre-sample treatment and logistics
  • Your samples are sequenced on the industry leading NovaSeq 6000 to reduce time and costs
  • You receive an interactive analysis report with t-SNE cluster plots and fold change tables
  • Expert bioinformaticians provide you with full support on comparative data-analysis
  • Single cell expression profiling is an ISO/IEC 17025 and ISO 15189 accredited service
Plaatje Anita vd Heuvel Single cell RNA seq
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Reduced Representation & Whole Genome Bisulfite Sequencing

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Single cell RNA-seq

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Targeted methylation

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MethylationEPIC ‘850K’ BeadChip

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EpiRestore: restore all low integrity DNA