qBiCo: A novel tool for assessing bisulfite conversion in epigenetics.
A novel tool for assessing bisulfite conversion in epigenetics.
Netherlands Organisation for Scientific Research (NWO) – Grant ID 18560
1 March 2021 to 31 August 2022
Bisulfite conversion of genomic DNA is considered to be the gold standard in epigenetic analysis. It is being used extensively for not only epigenetic marker discovery in medical research (e.g. epidemiology, aging, development, oncology) but also for the development of targeted epigenetic assays (e.g. diagnostics and forensics). Despite this method’s great power and applicability, a major disadvantage is that users of bisulfite conversion kits need to assume its conversion and DNA recovery rates. Erasmus MC has developed a new tool – qBiCo – a first-of-its-kind, multiplex, TaqMan-based quantitative PCR (qPCR) assay that can simultaneously assess the concentration, conversion rate, fragmentation and potential inhibition of a bisulfite-converted DNA sample. It can be applied in all sodium bisulfite-based epigenetic applications. At present, researchers are looking into introducing the tool to the market. Within the project, GenomeScan participates in the user committee meetings providing an expertise in the Next Generation Sequencing.
T.b.a. (ongoing project)
This work is part of the NWO research programme with project number 18560, which is (partly) funded by the Dutch Research Council (NWO).