MitochondrialScan

Enrich for mitochondrial DNA

Enrichment for mitochondrial DNA: Capturing and removal of methylated nucleotides from total DNA  

With MitochondrialScan we offer a product that enables you to effectively study mitochondrial diseases.

MitochondrialScan allows you effectively study mitochondrial DNA (mtDNA) in all organisms. This test allows you to study genes that code for metabolic diseases in humans as well as mtDNA that codes for specific traits in plants. With MitochondrialScan you receive a report on all unmethylated DNA present in the cell. The biggest advantage of this test is that you send in the total DNA sample without the need to isolate or purify the mitochondria.

MitochondrialScan is a technically advanced and cost-effective alternative for mitochondrial isolation kits. These kits usually require additional PCR cycles to increase the purity. MitochondrialScan is performed on the whole DNA fraction and offers an unbiased analysis of the total genetic variety of mitochondrial sequences in the samples.

What is MitochondrialScan?
GenomeScan provides a full service product from entry QC to data-analysis. We start the workflow with total DNA that we subject to an extensive quality control. By removing all methylated DNA from the sample, mitochondrial DNA (and for plants: chloroplast DNA) are specifically enriched. Removing genomic DNA reduces the library size extensively so that sequencing costs are lowered.

Our experienced bioinformatics experts have developed a validated pipeline that gives you a detailed overview of the variants compared to reference mtDNA. You receive an overview of the results in a comprehensive report. For the analysis and interpretation of patient mtDNA, get in touch with our scientific sales support.

Advantages of MitochondrialScan:

  • No prior  isolation of mtDNA required
  • Applicable to all organisms (micro-organisms, plants, humans)
  • Technically superior to mtDNA isolation kits
  • Sharp pricing due to sequencing of the relatively small mtDNA chromosome
  • Allows studying the phylogeny or relatedness of populations, since the mtDNA evolves faster than nuclear genetic markers
  • Data-analysis with comprehensive reporting
Which genes lie on the mitochondrial DNA?

The 37 genes of mtDNA, all code for proteins that are essential for normal mitochondrial function. Thirteen of these genes code for enzymes involved in oxidative phosphorylation, which generates ATP from glycogen and oxygen. The remaining genes code for transfer RNA (tRNA) and ribosomal RNA (rRNA), which are involved in gene translation.

The mtDNA genes are implicate in many diseases such as Parkinson, Kearns-Sayre syndrome (KSS), MELAS, MERFF and many others.

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A GenomeScan brand

ServiceXS is our research department for genetic analyses. Our scientists constantly test and develop technologies and applications to stay one step ahead in genetic testing. We offer a wide range of applications for veterinary, agriculture, microbial and medical research.