MethylationEPIC '850K'

Highly popular Epigenetic BeadChip

Our human methylation profiling service

The MethylationEPIC ‘850K’ has all the benefits of its predecessor (450K BeadChip), and offers double the amount of interrogated loci. Experts in the field have updated the content, so even recently described clinically relevant methylation sites are incorporated. This array combines a unique wealth of data with low costs per sample and easy interpretation.

Illumina’s MethylationEPIC ‘850K’ BeadChip is an excellent tool for quantitative methylation measurement at single CpG site level. It allows you to interrogate over 850,000 methylation sites, selected by experts in the field. The results give an overview of the total methylome, which can be used for Epigenome-Wide Association Studies (EWAS) on various human tissues.

Content of the MethylationEPIC BeadChip:

  • Combination of Coding and Enhancer regions
  • CpG sites outside of CpG islands
  • Differentially methylated sites identified in tumor vs. normal
  • Non-CpG methylated sites identified in human stem cells (CHH sites)
  • miRNA promoter regions

Quality inspection

Our automated and robust methylation workflow results in an unprecedented high concordance between arrays.
The correlation between technical replicates is generally above 0.99. That is why we guarantee to report at least 95% of all loci present on the MethylationEPIC BeadArray.
Highly reproducible data between both BeadChips and experiments is especially important when identifying small epigenetic changes or when multiple experiments must be combined, such as in cohort studies.

Benefits of choosing GenomeScan:

  • Full service, including automated DNA isolation for FFPE
  • QC measurements at multiple stages to tightly monitor sample quality
  • Highly specialized researchers perform the experiments leading to unprecedented reproducibility
  • High throughput and automation leads to cohort-friendly pricing
  • Excellent scientific advice in experimental design
  • One project manager that keeps you informed of the experiments at every stage of the project
The Infinium MethylationEPIC BeadChip employs both Infinium I and Infinium II assays. Infinium I assay design employs 2 bead types per CpG locus, 1 each for the methylated and unmethylated states. The Infinium II design uses 1 bead type, with the methylated state determined at the single base extension step after hybridization.
Application Restoration

required

Percentage of detected CpGs guaranteed Min. no. of detected CpGs For number of samples
Methylation No 95 % 807,500 100 %
FFPE Methylation Yes 90 % 765,000 80 %
Hydroxymethylation No 95 % 807,500 100 %
FFPE Hydroxymethylation Yes 90 % 765,000 80 %

Reduced Representation & Whole Genome Bisulfite Sequencing

Hydroxymethylation

Targeted methylation

MethylationEPIC ‘850K’ BeadChip

EpiRestore: restore all low integrity DNA