Customizable NGS-based solutions for pharma, biotech, and academic institutions

We offer innovative NGS and microarray-based solutions for genetic research and diagnostics

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The major benefits of working with GenomeScan:

People 01 GenomeScan

While sharing our knowledge and experience, we keep listening to understand your constraints and your needs. Our commitment and dedication keep us on your team long after completion of your project with excellent customer care.

Experience 01 GenomeScan

With scientists and technicians from various disciplines, we broaden our network. They can be considered as extra members of your team.

Innovation 01 GenomeScan

Our close contacts with technology providers and numerous collaborations with academic facilities give you access to the most advanced sequencing methodologies.

Quality 01 GenomeScan

We are committed to complete your project on time, on budget and according to stringent quality criteria.

GenomeScan offers customizable sequencing solutions covering a great variety of DNA and RNA applications:

You can find more information on these applications in the DNA and RNA tabs. Alternatively, you can download the Sample Submission Guide with an overview of key information to initiate your project.

All samples should be submitted in ≥ 15 μl volume

Application Input material Optimal amount (per sample) Concentration range Turnaround time*
Whole Genome Sequencing (WGS) Purified gDNA Standard: >10 ng
 PCR free protocol: >100ng 5-50 ng/μl 2-3 weeks
Targeted Resequencing Purified gDNA/ Amplicon Standard: >10 ng 
PCR free protocol: >100ng 5-50 ng/μl 2-3 weeks
Whole Exome Sequencing (WES) Purified gDNA 500 ng 50-200 ng/μl 2-3 weeks
ChIP-Sequencing/complexity reduction Enriched DNA Standard: >10 ng
PCR free protocol: >100ng >2 ng/μl 2-3 weeks
Whole Genome Bisulfite Sequencing (WGBS) Purified gDNA 100 ng >10 ng/μl 3-4 weeks
Mitochondrial DNA (mtDNA) Purified gDNA 250-500 ng 5-50 ng/μl 2-3 weeks
Microbiome16S rRNA Sequencing Purified gDNA 1-100 ng ** >2 ng/μl 2-3 weeks
Whole Microbiome Sequencing Purified gDNA 250-500 ng 5-50 ng/μl 2-3 weeks
Methylation EPIC BeadChip (850K) Purified gDNA 750-1000 ng 75-100 ng/μl 4-6 weeks
PacBio Sequencing Purified gDNA/ Amplicon 500 ng- 5μg >50 ng/μl 2-6 weeks

* Turnaround time (TAT) depends on project size and starts after successful sample

** Depending on sample origin

All samples should be submitted in ≥ 15 μl volume

Application Input material Optimal amount (per sample) Concentration range Turnaround time*

RNA-Seq using Poly-A selection ** (Gene-expression profiling)

Purified RNA

≥100 ng

10-50 ng/μl

3-4 weeks

RNA-Seq using rRNA reduction ** (Total transcriptome)

Purified RNA

≥ 30 ng

3-50 ng/μl

3-4 weeks

Small RNA-Seq

Purified RNA or equivalent of enriched small RNA

1000 ng or enriched equivalent

250-350 ng/μl

3-4 weeks

Low-input RNA-Seq

Purified RNA (or cells)

1 ng

>0.1 ng/μl

3-4 weeks

Single cell RNA-Seq

Methanol fixed cells

1.000 cells

1.000-10.000 cells

3-4 weeks

* Turnaround time (TAT) depends on project size and starts after successful sample QC

** Optimal input for RNA-Seq based on 30% or less PCR duplicates

The table below specifies the input requirement for RNA samples. Be aware that these recommendations were determined using a high purity and quality reference sample. Samples of sub-optimal quality will require more material to achieve similar numbers.

Input Optimal Recommended Minimum

Poly-A selection (Gene expression profiling)

≥ 100 ng

≥ 30 ng

1 ng

 

rRNA depletion (Total transcriptome)

≥ 30 ng

≥ 10 ng

0.5 ng

Expected duplicates

< 30%

30 – 50%

> 80%

 

+
Our Services

GenomeScan offers customizable sequencing solutions covering a great variety of DNA and RNA applications:

You can find more information on these applications in the DNA and RNA tabs. Alternatively, you can download the Sample Submission Guide with an overview of key information to initiate your project.

+
DNA

All samples should be submitted in ≥ 15 μl volume

Application Input material Optimal amount (per sample) Concentration range Turnaround time*
Whole Genome Sequencing (WGS) Purified gDNA Standard: >10 ng
 PCR free protocol: >100ng 5-50 ng/μl 2-3 weeks
Targeted Resequencing Purified gDNA/ Amplicon Standard: >10 ng 
PCR free protocol: >100ng 5-50 ng/μl 2-3 weeks
Whole Exome Sequencing (WES) Purified gDNA 500 ng 50-200 ng/μl 2-3 weeks
ChIP-Sequencing/complexity reduction Enriched DNA Standard: >10 ng
PCR free protocol: >100ng >2 ng/μl 2-3 weeks
Whole Genome Bisulfite Sequencing (WGBS) Purified gDNA 100 ng >10 ng/μl 3-4 weeks
Mitochondrial DNA (mtDNA) Purified gDNA 250-500 ng 5-50 ng/μl 2-3 weeks
Microbiome16S rRNA Sequencing Purified gDNA 1-100 ng ** >2 ng/μl 2-3 weeks
Whole Microbiome Sequencing Purified gDNA 250-500 ng 5-50 ng/μl 2-3 weeks
Methylation EPIC BeadChip (850K) Purified gDNA 750-1000 ng 75-100 ng/μl 4-6 weeks
PacBio Sequencing Purified gDNA/ Amplicon 500 ng- 5μg >50 ng/μl 2-6 weeks

* Turnaround time (TAT) depends on project size and starts after successful sample

** Depending on sample origin

+
RNA

All samples should be submitted in ≥ 15 μl volume

Application Input material Optimal amount (per sample) Concentration range Turnaround time*

RNA-Seq using Poly-A selection ** (Gene-expression profiling)

Purified RNA

≥100 ng

10-50 ng/μl

3-4 weeks

RNA-Seq using rRNA reduction ** (Total transcriptome)

Purified RNA

≥ 30 ng

3-50 ng/μl

3-4 weeks

Small RNA-Seq

Purified RNA or equivalent of enriched small RNA

1000 ng or enriched equivalent

250-350 ng/μl

3-4 weeks

Low-input RNA-Seq

Purified RNA (or cells)

1 ng

>0.1 ng/μl

3-4 weeks

Single cell RNA-Seq

Methanol fixed cells

1.000 cells

1.000-10.000 cells

3-4 weeks

* Turnaround time (TAT) depends on project size and starts after successful sample QC

** Optimal input for RNA-Seq based on 30% or less PCR duplicates

The table below specifies the input requirement for RNA samples. Be aware that these recommendations were determined using a high purity and quality reference sample. Samples of sub-optimal quality will require more material to achieve similar numbers.

Input Optimal Recommended Minimum

Poly-A selection (Gene expression profiling)

≥ 100 ng

≥ 30 ng

1 ng

 

rRNA depletion (Total transcriptome)

≥ 30 ng

≥ 10 ng

0.5 ng

Expected duplicates

< 30%

30 – 50%

> 80%

 

Let’s get the conversation started for your next NGS project.

Group 123 GenomeScan
Please either fill in this form or email us directly at info@genomescan.nl
and we will get in touch with you to discuss your requirements.