31 October 2019 in Blog, News

Prostate Cancer Research Network (TransPot)

Translational Research Network for Prostate Cancer (TransPot) Today starts Movember (a combination of the English diminutive word for moustache, “mo”, and “November”), an annual event involving the growing of moustaches during the month of November to raise awareness of men’s health issues, such as prostate cancer, testicular cancer and men’s suicide. Prostate cancer is one of the most prevalent cancer…

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31 October 2019 in Blog

Five advantages of UMIs in DNA analysis

Improvements in Gene Panel and Whole Exome sequencing Molecular barcoding refers to the attachment of a unique molecular sequence (eg. ATGCCA) to each individual DNA fragment in a sample. This ensures that in downstream analysis, unique sequence reads can be separated from those originating as a result of PCR duplication. A common practice regarding these PCR duplicates is to search…

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24 October 2019 in Blog

Four reasons why UMIs improve gene expression profiling

Transcription profiles give accurate images of the biological processes that influence cell fates. However, library construction can introduce bias at multiple steps. For example, PCR amplification increases the number of cDNA molecules to an amount sufficient for sequencing, but also stochastically introduces errors and amplifies different molecules with unequal probabilities. The sequencing reads that have been created from the same…

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21 October 2019 in Blog

How to solve major sequencing pitfalls with Unique Molecular Identifiers (UMIs)

If you start analyzing your gene-expression data set, many questions come to mind that can seriously affect the outcome. What if any of the PCR steps during library prep (Figure 1) has introduced an error? It might have led to a base change, which I could incorrectly score as a mutation. Did a PCR-bias favor amplification of some of the…

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Five advantages of UMIs in DNA analysis

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Improvements in Gene Panel and Whole Exome sequencing Molecular barcoding refers to the attachment of a unique molecular sequence (eg. ATGCCA) to each individual DNA fragment in a sample. This ensures that in downstream analysis, unique sequence reads can be separated from those originating as a result of PCR duplication. A common practice regarding these PCR duplicates is to search…

Read More
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Four reasons why UMIs improve gene expression profiling

By | Blog | No Comments

Transcription profiles give accurate images of the biological processes that influence cell fates. However, library construction can introduce bias at multiple steps. For example, PCR amplification increases the number of cDNA molecules to an amount sufficient for sequencing, but also stochastically introduces errors and amplifies different molecules with unequal probabilities. The sequencing reads that have been created from the same…

Read More