RRBS and WGBS

Illumina solely provides methylation arrays for human samples. Selection of a custom approach is also necessary for those interested in CpGs outside Illumina’s 850K target list, or when a broader overview of the methylome is required. Our scientists have selected a targeted sequencing approach, that enriches for CpG rich areas. It employs the MspI enzyme to digest genomic DNA at C^CGG motives. Small fragments of methylated DNA can specifically be selected from the whole genome sample, thereby reducing the complexity of the genome. Discarding regions without methylated residues leads to lower sequencing costs, due to the reduction in the size of the library that must be sequenced. RRBS therefore combines the wealth of unbiased data that only Next Generation Sequencing can provide, with relatively low costs.